John Constantine (DC Comics) an occult detective and professional sorcerer from Liverpool, England.
Gabriel Van Helsing (Van Helsing) an expert in dealing with, among other things, vampires, gargoyles, and warlocks.
Sam Winchester (Supernatural) was trained along with his brother as a hunter from an early age, and is just as capable as him of taking on supernatural beings.
Having been trained as a hunter from an early age, Dean Winchester (Supernatural) is more than capable of holding his own against supernatural beings.
(right to left) Ray Stantz, Peter Venkman, Egon Spengler, and Winston Zeddemore (Ghostbusters)
After his brother was captured by ghosts, Luigi (Luigi's Mansion) proved his courage by going into the haunted mansion to rescue him, armed with his trusty Poltergust 3000 to help him capture ghosts.
Though a mere human, Sango (InuYasha) was trained as a demon slayer from an early age and is more than capable of handling different species of demons.
Grimms (Grimm) are people who possess powers, such as being able to see the true form of Wesen. For centuries, they have policed and hunted the Wesen population to protect normal humans from the unknown threats.
The Bureau for Paranormal Research and Defense/B.P.R.D. (Hellboy) is an organization charged with protecting America and the world from the occult, paranormal and supernatural. It maintains the services of several supernatural persons, including Hellboy.
Shiki Tohno (TYPE-MOON)
Gwen Tennyson (Ben 10)
Elsa Bloodstone (Marvel Comics)
Doctor Stephen Strange (Marvel Comics)
Eric Brook (Marvel Comics), commonly known as Blade.