Gabriel Van Helsing (Van Helsing) is an expert in dealing with, among other things, vampires, gargoyles, and warlocks.
Having been trained as a hunter from an early age, Dean Winchester (Supernatural) is more than capable of holding his own against supernatural beings.
Sam Winchester (Supernatural) was trained along with his brother as a hunter from an early age, and is just as capable as him of taking on supernatural beings.
(right to left) Ray Stantz, Peter Venkman, Egon Spengler, and Winston Zeddemore (Ghostbusters)
Luigi and his Poltergust (Luigi's Mansion)
Though a mere human, Sango (InuYasha) was trained as a demon slayer from an early age and is more than capable of handling different species of demons.
John Constantine (DC Comics) is an occult detective and professional sorcerer from Liverpool, England.
Grimms (Grimm) are people who possess powers, such as being able to see the true form of Wesen. For centuries, they have policed and hunted the Wesen population to protect normal humans from the unknown threats.
The Bureau for Paranormal Research and Defense/B.P.R.D. (Hellboy) is an organization charged with protecting America and the world from the occult, paranormal and supernatural. It maintains the services of several supernatural persons, including Hellboy.