The Superman of the Kingdom Come universe (DC Comics) has developed an immunity to Kryptonite.
As a "neo-vampire," Hannibal King (Marvel Comics) is resistant to sunlight and has only a craving for blood instead of an actual need for it.
As a dhampir, Blade (Marvel Comics) has all of the strengths and abilities of the vampire race with none of the weaknesses except for their thirst.
As a Lycan/vampire hybrid, Michael Corvin (Underworld) is immune to both species' trademark weaknesses.
As a vampire/Corvinus Strain hybrid, Selene (Underworld) is unharmed by sunlight.
As a dhampir, Rayne (BloodRayne) is immune to holy items and resistant to sunlight and water.
Due to his advanced age and strength, Spike (Buffy the Vampire Slayer) is more resistant to sunlight and holy items than most vampires.
Due to his advanced age and strength, Angel (Buffy the Vampire Slayer) is more resistant to sunlight and holy items than most vampires.
As a human-Kryptonian hybrid, Superboy (DC Comics) is more resistant to Kryptonite than pure-blood Kryptonians.
Katherine Pierce (The Vampire Diaries) has developed an immunity to vervain by repeatedly ingesting it in trace amounts.
Having been created through a combination of Superman's DNA and Kryptonite, Superman X (Legion of Super-Heroes) is immune to Kryptonite.
The Reapers (Blade II) are immune to all conventional vampire weaknesses except sunlight
As the original Reaper, Jared Nomak has far better control over his blood-thirst and retains all of his higher brain functions.
Alucard (Hellsing) is immune to all vampire weaknesses except the most holy of Christian artifacts, such as Helena's Nail.
The enhanced Type 2 Las Plagas (Resident Evil) were able to tolerate direct sunlight, though flash grenades were still fatal to them.
Alex Mercer (Prototype) developed an immunity to the anti-Blacklight poison Bloodtox with repeated exposure.
Daniel "Oz" Osbourne (Buffy the Vampire Slayer) has been able to control his werewolf transformations through meditation and herbal remedies.
Kain (Legacy of Kain) has developed immunity to sunlight and certain forms of water (rain, snow, etc.)
Saya Otonashi (Blood+) has been able to control her blood-thirst through regular blood transfusions and eating large quantities of human food.
Quint Lane (Underworld: Awakening) has incredible resistance to silver as a Lycan-Corvinus Strain hybrid, but a silver nitrate grenade going off inside his body was enough to kill him.
When he still had a tail, Vegeta (Dragon Ball) was able to overcome the Saiyans' traditional weakness of having their tail squeezed.
Goku (Dragon Ball) overcame his weakness to his tail being squeezed through extensive training.
Thanks to the second skin provided by the Omnitrix, Ghostfreak (Ben 10) can venture out into the sun without harm.
Daurgothoth, the creeping doom (Forgotten Realms) has transcended not only the limits of his undead body, but also the limits of the dragons species as a whole, developing new breath attacks that he shouldn't be able to use, as well as developing other abilities that should be impossible for him because of his undead status.
The Frog Hermit (Ranma 1/2) has transcended the limits of his jusenkyo curse, allowing him to talk and fight as if he was still in his human form.
As he was originally human and turned into a vampire, Tsukune Aono (Rosario + Vampire) is unaffected by the traditional vampire vulnerability to water.
After entering Gear Fourth, Luffy (One Piece) is able to retain his natural rubber state despite being coated completely with his Haki which should normally neutralize it, allowing attacks that would normally harm him to simply bounce off.
As he remains God's chosen one despite being a vampire, Gabriel Belmont/Dracula (Castlevania: Lords of Shadow 2) is unaffected by the traditional vampire vulnerability to holy items like crosses, to the extent that he can physically touch one without harm.
Dark Sister (Valkyrie Crusade) is a Vampire that is immune to all of an vampire's weaknesses, such as sunlight and holy powers and objects.
Due to its unique composition, the Crystal Onix (Pokémon) is immune to water and Water-type attacks, unlike other Onix.
After being implanted with the Mantra Reactor, Asura (Asura's Wrath) is able to freely channel his Wrath Mantra without fear of it backfiring and destroying his body.
By consuming Rahab's soul, Raziel (Legacy of Kain) was able to overcome his vulnerability to water.